Re: [ccp4bb] An alternative to glycerol in keeping protein soluble?

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: An alternative to glycerol in keeping protein soluble?
From: SERAH KIMANI SERAH {- dot -} KIMANI {- at -} UCT {- dot -} AC {- dot -} ZA
Date: 2010-02-23

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Subject: Re: An alternative to glycerol in keeping protein soluble?
From: Annie Hassell annie {- dot -} m {- dot -} hassell {- at -} GSK {- dot -} COM
Date: 2010-02-23

Subject: Re: An alternative to glycerol in keeping protein soluble?
From: Puneet juneja junejabiotech {- at -} GMAIL {- dot -} COM
Date: 2010-02-23

In general

Osmotropes, chaotropes, amino acid, sugar , polyhydric alcohol,
detergents..... promote protein solubility

Puneet Juneja

On Tue, Feb 23, 2010 at 12:07 PM, SERAH KIMANI wrote:

> Dear all,
>
> Does anyone have an idea of something else that I can use instead glycerol
> to maintain solubility of my protein? I have been having 10% glycerol in my
> protein solution and this has helped me get crystals in like three different
> conditions. However, I would want to get crystals of mutants-substrate
> complexes, but unfortunately glycerol interferes with the binding of the
> substrates to my protein. So, for the complexes to form, glycerol has to be
> out (I have tested this using the wildtype enzyme both in the presence and
> absence of glycerol). Now, in the absence of glycerol, I get a lot of
> precipitation, and no crystals even after optimizing around the known
> conditions with different protein concentrations. I have tried re-screening
> for new conditions in the absence of glycerol, but I haven't found any
> condition that yields crystals in the absence of glycerol.
>
> I was wondering if anyone might know of something else that I could use in
> place of glycerol in my protein solution??
>
> Regards,
>
> Serah
> University of Cape Town
>
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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: An alternative to glycerol in keeping protein soluble?
From: SERAH KIMANI SERAH {- dot -} KIMANI {- at -} UCT {- dot -} AC {- dot -} ZA
Date: 2010-02-23

Next message:
Subject: Re: An alternative to glycerol in keeping protein soluble?
From: Annie Hassell annie {- dot -} m {- dot -} hassell {- at -} GSK {- dot -} COM
Date: 2010-02-23