Quick navigation: Home   |    Site Map   ||    References   |    Biography   ||    Copyright   |    Other copyright   |    Contact us   |    Advert   |   
 

Re: [ccp4bb] Did anyone here know how to get the Rfree value of high resolution range
- Protein crystallography

Main steps:

   - Protein purification
   - Crystallisation

Special:

   - Programs for crystallography
   - X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

   - CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Re: units of f0, f', f''
From: Gerard Bricogne gb10 {- at -} GLOBALPHASING {- dot -} COM
Date: 2010-02-27		Next message:
Subject: Re: units of f0, f', f''
From: Ian Tickle I {- dot -} Tickle {- at -} ASTEX-THERAPEUTICS {- dot -} COM
Date: 2010-02-27


Subject: Re: Did anyone here know how to get the Rfree value of high resolution range
From: Boaz Shaanan bshaanan {- at -} BGU {- dot -} AC {- dot -} IL
Date: 2010-02-27

Hi,

And just an additional (trivial) remark to that of Tim (in case you missed that): make sure you have a -1 in the second place on the CGLS card like in:

CGLS 10  -1

Otherwise, as far as I know, Rfree won't get printed at all.

 Cheers,

              Boaz
----- Original Message -----
From: Tim Gruene
Date: Saturday, February 27, 2010 11:48
Subject: Re: [ccp4bb] Did anyone here know how to get the Rfree value of high resolution range
To: CCP4BB@JISCMAIL.AC.UK

> Dear Yuan,
>
> I suppose you refer to a shelxl refinement?
> The Rfree is listed in the lst-file, too. Go to the bottom of
> that file and
> search backwards  for the word "Free". Make sure you pick
> up the second value in
> that row, the one for all reflections, not just the strong one.
>
> I do not think there is a listing "Rfree/resolution shell", for
> as much as I
> understand that won't make much sense generally: For Rfree to be
> meaningful you
> require at least 500 reflections and you probably do not have
> 500 reflections
> flagged for Rfree in the outer resolution shell.
>
> I hope that last sentence is not a misconception of mine.
>
> Tim
>
> On Sat, Feb 27, 2010 at 05:13:15AM +0000, Yuan SHANG wrote:
> > Hi,all,
> > I can get the R-value~resolution statistics from .lst file,but
> where is
> > the Rfree value?
> >
> >
> > Thanks in advance.
> >
> > Yuan
>
> --
> --
> Tim Gruene
> Institut fuer anorganische Chemie
> Tammannstr. 4
> D-37077 Goettingen
>
> GPG Key ID = A46BEE1A
>
>

Boaz Shaanan, Ph.D.
Dept. of Life Sciences
Ben-Gurion University of the Negev
Beer-Sheva 84105
Israel
Phone: 972-8-647-2220 ; Fax: 646-1710
Skype: boaz.shaanan‎
ProteinCrystallography.org: Copyright 2006-2010 by Quid United Ltd