Re: [ccp4bb] Partial binding of ligand to multimer

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Partial binding of ligand to multimer
From: Michal Harel michal {- dot -} harel {- at -} WEIZMANN {- dot -} AC {- dot -} IL
Date: 2007-06-24

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Subject: Re: stereo, dual head, CRT and TFT, Xorg.config, linux
From: Jeroen Mesters mesters {- at -} BIOCHEM {- dot -} UNI-LUEBECK {- dot -} DE
Date: 2007-06-25

Subject: Re: Partial binding of ligand to multimer
From: Miguel_Ortiz-Lombardía ibdeno {- at -} GMAIL {- dot -} COM
Date: 2007-06-24

Hi Michal,

Hmmm... Was it kicked out upon crystallisation or was crystallisation
possible because most of your pentamers were not fully occupied by ligand so
allowing the possibility of good contacts? ;-)

What was the excess of ligand? Do you know its affinity?

Miguel

2007/6/24, Michal Harel :
>
> Hi all, We have a structure of a pentamer which was crystallized from
> a complex of the protein with a ligand. The structure shows that 4 out
> of 5 binding sites are occupied. The symmetry-related molecules pack
> much tighter around the unoccupied monomer. I am looking for examples
> of structures in which a ligand was "kicked out" of a binding site upon
> crystallization.
>
> Regards - Michal.
> ------------------------------------------------------------------------
> Dr. Michal Harel
> Dept. of Structural Biology michal.harel@weizmann.ac.il
> Weizmann Inst. of Science tel: 972-8-9342647
> Rehovot 76100, ISRAEL fax: 972-8-9344159
> ------------------------------------------------------------------------
>

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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Partial binding of ligand to multimer
From: Michal Harel michal {- dot -} harel {- at -} WEIZMANN {- dot -} AC {- dot -} IL
Date: 2007-06-24

Next message:
Subject: Re: stereo, dual head, CRT and TFT, Xorg.config, linux
From: Jeroen Mesters mesters {- at -} BIOCHEM {- dot -} UNI-LUEBECK {- dot -} DE
Date: 2007-06-25