Re: [ccp4bb] solve structure by molecular replacement

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: anomalous phase extension to native data
From: Jürgen_Bosch jubosch {- at -} JHSPH {- dot -} EDU
Date: 2010-03-08

Next message:
Subject: Re: anomalous phase extension to native data
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2010-03-08

Subject: Re: solve structure by molecular replacement
From: Jürgen_Bosch jubosch {- at -} JHSPH {- dot -} EDU
Date: 2010-03-08

Your molecule seems to be titled towards at least one of the axis, so there's no problem with your dimensions in P21.

Jürgen

On Mar 7, 2010, at 7:40 PM, Chunmin L wrote:

> Hi Jürgen,
>
> Many thanks for your message. Somehow Mosflm in CCP4 did not
> recognize the images. Will try XDS. or d*TREK. Still no answers for
> that P21 one-molecule solution. It does not collide with itself. The
> model is about 60 A wide and 82 A long. Unit cell dimensions of a=50
> b=51 c=252. if we have 82 A along the 252A, the 60 A is wider than
> the
> other two dimensions. I was surprised to see the previous postdoc got
> pretty good density for this molecule.
>
> Many thanks,
> Chunmin
>
> On Sun, Mar 7, 2010 at 3:00 PM, Jürgen Bosch wrote:
>>
>> On Mar 7, 2010, at 4:35 PM, Chunmin L wrote:
>>
>> I can not reprocess the data to
>> check the systematic absence to determine the space group (I do not
>> have the right software to reprocess the raw image at this time).
>>
>> What exactly do you mean by that ? The world does not consist of HKL alone,
>> there are other options available even CCP4 ones e.g. Mosflm or non-CCP4
>> e.g. XDS. or d*TREK
>> Jürgen
>> -
>> Jürgen Bosch
>> Johns Hopkins Bloomberg School of Public Health
>> Department of Biochemistry & Molecular Biology
>> Johns Hopkins Malaria Research Institute
>> 615 North Wolfe Street, W8708
>> Baltimore, MD 21205
>> Phone: +1-410-614-4742
>> Lab: +1-410-614-4894
>> Fax: +1-410-955-3655
>> http://web.mac.com/bosch_lab/
>>

-
Jürgen Bosch
Johns Hopkins Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Baltimore, MD 21205
Phone: +1-410-614-4742
Lab: +1-410-614-4894
Fax: +1-410-955-3655
http://web.mac.com/bosch_lab/

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: anomalous phase extension to native data
From: Jürgen_Bosch jubosch {- at -} JHSPH {- dot -} EDU
Date: 2010-03-08

Next message:
Subject: Re: anomalous phase extension to native data
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2010-03-08