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[ccp4bb] Squashed density |
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- Protein crystallographyMain steps:- Protein purification- Crystallisation Special:- Programs for crystallography- X-ray detectors Basic tutorials:- Chemistry- Protein - Peptide - Amino Acids Xtal community:- CCP4BB |
CCP4bb navigationCCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999Subject: Squashed density From: "Kolstoe S {- dot -} E {- dot -} " S {- dot -} E {- dot -} KOLSTOE {- at -} SOTON {- dot -} AC {- dot -} UK Date: 2007-06-27 Dear CCP4 message board, I am having a problem with electron density that looks as if it has been compressed along the Y axis. Density that would appear as a sphere looks like an ellipsoid, and the effect seems worse in some regions (typically where I am having problems building in a flexible loop). It is worse in my Fo-Fc map (compared to my 2Fo-Fc) which makes me wonder if there is a problem with my calculated F's? Does anyone know of common reasons for seeing this effect, and how to fix it? (I processed the data in Mosflm, scaled in Scala, phased in phaser and then used the usf program dataman to pick my R free set and convert to cns format, and have been using anneal, minimize and model_map in CNS). Simon CCP4bb navigationCCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999 |
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