Re: [ccp4bb] Butandiol as cryopotectant

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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From: Gerard Bricogne gb10 {- at -} GLOBALPHASING {- dot -} COM
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Subject: Re: Butandiol as cryopotectant
From: Eike Schulz eschulz1 {- at -} GWDG {- dot -} DE
Date: 2010-03-23

Subject: Re: Butandiol as cryopotectant
From: David Briggs drdavidcbriggs {- at -} GMAIL {- dot -} COM
Date: 2010-03-23

2,3-BD users,

What sort of concentrations are you using?

Dave

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On Mar 22, 2010 11:45 PM, "Steve Tuske" wrote:

Hi Ulrike,

2,3-butanediol has worked very nicely as a cryoprotectant. In my hands it
mostly seems to decrease mosaicity. I was able to switch from a
three-step soaking protocol with glycerol and glucose to a quick (30
second to one minute) soak with 2,3-BD.

It's a winner.

Steve

I

> Hello Ulrike, > > in our lab 2,3-Butanediol has had a good reputation. > >
Tim > > On Mon, Mar 22...

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Why Do Phases Dominate?
From: Gerard Bricogne gb10 {- at -} GLOBALPHASING {- dot -} COM
Date: 2010-03-22

Next message:
Subject: Re: Butandiol as cryopotectant
From: Eike Schulz eschulz1 {- at -} GWDG {- dot -} DE
Date: 2010-03-23