Re: [ccp4bb] update on SeMet production

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: SUMMARY: update on SeMet production
From: Tim Gruene tg {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2010-03-23

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Subject: Re: How to upload ligand library in win coot
From: Paul Emsley paul {- dot -} emsley {- at -} BIOCH {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2010-03-23

Subject: Re: update on SeMet production
From: Ronnie Berntsson r {- dot -} berntsson {- at -} RUG {- dot -} NL
Date: 2010-03-23

Hi Tim,

Lactococcus lactis can also produce SeMet proteins, see:
Berntsson et al. Selenomethionine incorporation in proteins expressed in Lactococcus lactis. Protein Sci (2009) vol. 18 (5) pp. 1121-7

Cheers,
Ronnie

On Mar 23, 2010, at 14:40, Tim Gruene wrote:

> Dear all,
>
> I already received a couple of replies.
>
> * mammalian cells:
> http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2242577/pdf/2008.pdf
> mentions several mammalian cell lines, including CHO, HEK239, and COS.
> Apparently, Lec8 have been used, too.
> * Yeast
> * stable S2 insect cells
> * baculovirus expression system
> * in addition to E.coli, P. aeruginosa was also mentioned.
>
> Thanks to everyone who replied.
>
> Tim
>
>
> On Tue, Mar 23, 2010 at 01:25:56PM +0100, Tim Gruene wrote:
>> Dear all,
>>
>> since I am currenty preparing a lecture on crystallography I am wondering about
>> the status quo of the production of SeMet proteins.
>> In 2003, if I remember correctly, it was possible to express SeMet proteins in
>> E.coli and insect cells.
>>
>> Has this been extended to other systems, and if so, which ones?
>>
>> Thanks a lot,
>> Tim
>>
>> --
>> --
>> Tim Gruene
>> Institut fuer anorganische Chemie
>> Tammannstr. 4
>> D-37077 Goettingen
>>
>> GPG Key ID = A46BEE1A
>>
>
>
>
> --
> --
> Tim Gruene
> Institut fuer anorganische Chemie
> Tammannstr. 4
> D-37077 Goettingen
>
> GPG Key ID = A46BEE1A
>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: SUMMARY: update on SeMet production
From: Tim Gruene tg {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2010-03-23

Next message:
Subject: Re: How to upload ligand library in win coot
From: Paul Emsley paul {- dot -} emsley {- at -} BIOCH {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2010-03-23