Re: [ccp4bb] heavyatom soaking problem

- Protein crystallography

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: compiling problems ccp4-6.0.2 and gcc-4.1.2
From: Tim Grune tim {- dot -} grune {- at -} SYNCHROTRON {- dot -} VIC {- dot -} GOV {- dot -} AU
Date: 2007-06-28

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Subject: MSGPP cocktails for fragment crystallography
From: Jan Abendroth janabend {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2007-06-28

Subject: Re: heavyatom soaking problem
From: verdino {- at -} SCRIPPS {- dot -} EDU verdino {- at -} SCRIPPS {- dot -} EDU
Date: 2007-06-28

Hi,

give Ta6Br12 a try - it is a cluster with an incredible white line -
really nice for phasing :-). But the best thing is that it does not mind
high ionic strength (worked great for me). There are a couple of
references out for it which you can check for more details...

Good luck, Petra

> Hello all,
> I am struggling with getting the phases out for my protein. Heavyatom
> database shows that only mercury and samarium have the binding motifs in
> it.
>
> Sodium iodide soak is killing the crystal even as less as 0.2 Molar
> concentration for 30sec.
> With Potassium iodide crystal is okay upto 0.75M for 1 & a half min but
> the
> incorporation is not at relevent sites.(Iodide signal is not distinct and
> is weak)
> Hg & Au salts precipitate it.
> With Pt signal is weak.
> Somewhere I read that high salt causes problems for heavy atom to bind to
> protein.My protein needs high salt for stability (750mM NaCl)
>
> Please help with some suggestion!!
>

--
Petra Verdino, PhD
Research Associate
The Scripps Research Institute, BCC206
10550 North Torrey Pines Road
CA 92037 La Jolla, USA
tel:1-858-784-2294
fax:1-858-784-2980

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: compiling problems ccp4-6.0.2 and gcc-4.1.2
From: Tim Grune tim {- dot -} grune {- at -} SYNCHROTRON {- dot -} VIC {- dot -} GOV {- dot -} AU
Date: 2007-06-28

Next message:
Subject: MSGPP cocktails for fragment crystallography
From: Jan Abendroth janabend {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2007-06-28