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Re: [ccp4bb] packing
 

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CCP4bb <-- 2007 <-- June 2007 <-- 29 June 2007
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Subject: Re: heavyatom soaking problem
From: "Sanishvili, Ruslan" rsanishvili {- at -} ANL {- dot -} GOV
Date: 2007-06-29 		Next message:
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Subject: Re: packing
From: Tassos Papageorgiou apapageo {- at -} BTK {- dot -} UTU {- dot -} FI
Date: 2007-06-29
Dear all,

Thank you for your replies so far. I feel I should have been more precise in my
original question. There is a dimer in the asymmetric unit and we end up with
two different dimers after molecular replacement (the monomer-monomer interface
is completely different). Each solution refines with the respective data set.

Tassos

Quoting Wim Burmeister :

> Tassos Papageorgiou wrote:
> > Dear all,
> >
> > I am looking for examples of different packing arrangements in crystals of
> the
> > same protein with similar unit cell parameters and space group (eg the
> protein
> > adopts different packing although the crystals remain the same both in unit
> > cell dimensions and space group). Can this happen, for example, during
> flash
> > cooling?
> >
> > Thank you in advance
> >
> > Tassos Papageorgiou
> >
> >
> >
> > --
> > A.C.(Tassos) Papageorgiou, PhD phone: +358 2 333 8012 (office)
> > Senior Scientist, Group leader fax: +358 2 333 8000
> > Turku Centre for Biotechnology E-mail: apapageo@btk.utu.fi
> > BioCity, Turku URL:
> http://www.btk.utu.fi/~apapageo
> > FIN-20521, Finland
> >
> >
> >
> >
> Dear Tassos,
>
> I would guess that you rather have a problem of different choices of
> origins between your datasets which can happen for a number of
> spacegroups. This can make that the packing appears different on the
> first glimpse, but you should be able to translate one arrangement on
> top of another.
>
> Yours
>
> Wim
>
> --
>
*******************************************************************************
> Wim Burmeister
> Professeur, Membre de l'Institut Universitaire de France
> Unit of Virus Host Cell Interactions (UVHCI) UMR5233 UJF-EMBL-CNRS
> 6 rue Jules Horowitz
> B.P. 181, F-38042 Grenoble Cedex 9 FRANCE
> E-mail: wpb@embl-grenoble.fr
> Tel: +33 (0) 476 20 72 82 Fax: +33 (0) 476 20 94 00
> http://www2.ujf-grenoble.fr/pharmacie/laboratoires/gdrviro
>
*******************************************************************************
>




--
A.C.(Tassos) Papageorgiou, PhD phone: +358 2 333 8012 (office)
Senior Scientist, Group leader fax: +358 2 333 8000
Turku Centre for Biotechnology E-mail: apapageo@btk.utu.fi
BioCity, Turku URL: http://www.btk.utu.fi/~apapageo
FIN-20521, Finland
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