[ccp4bb] phosphate v sulfate

- Protein crystallography

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- Protein purification
- Crystallisation

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- X-ray detectors

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: freezing crystals grown in isopropanol condition
From: Patrick Shaw Stewart patrick {- at -} DOUGLAS {- dot -} CO {- dot -} UK
Date: 2010-04-10

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Subject: Re: phosphate v sulfate
From: Dhirendra K Simanshu simanshu {- at -} GMAIL {- dot -} COM
Date: 2010-04-10

Subject: phosphate v sulfate
From: wtempel wtempel {- at -} GMAIL {- dot -} COM
Date: 2010-04-10

Dear colleagues,
I would like to poll the community on the prevailing practice of
distinguishing between phosphate and sulfate in their structures.
Suppose all of the following apply:
1) a well contoured tetrahedral density in your model-phased 2Fo-Fc map in
the active site of your kinase or GTPase protein.
2) a significant, say 5*sigma, coincident peak in your model-phased
anomalous difference fourier map from data collected at a Cu rotating anode
source.
3) The crystal grew in 2M ammonium sulfate.
Please post your answers to the list if you feel this question is of general
interest.*
Thank you for your input.
Wolfram Tempel

*If it is not, I apologize to everyone for wasting their valuable time.

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: freezing crystals grown in isopropanol condition
From: Patrick Shaw Stewart patrick {- at -} DOUGLAS {- dot -} CO {- dot -} UK
Date: 2010-04-10

Next message:
Subject: Re: phosphate v sulfate
From: Dhirendra K Simanshu simanshu {- at -} GMAIL {- dot -} COM
Date: 2010-04-10