Re: [ccp4bb] Possible sulphate

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: CCP4BB Digest - 9 Apr 2010 to 10 Apr 2010 (#2010-97)
From: Matthew E Thornton thornton {- at -} CALTECH {- dot -} EDU
Date: 2010-04-11

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Subject: Re: Phasing statistics
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2010-04-12

Subject: Re: Possible sulphate
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2010-04-12

Rex Palmer wrote:
> What seems to be a possible sulphate has been identified in our electron density.
> What steps could/should be taken to confirm or consolidate this assignment that would satisfy referees?
>
> Rex Palmer
> Birkbeck College

If you place a sulphate and it refines in a sensible way - temperature
factors for the group more or less agreeing that is good.
Is there a decent set of H bonds?
Was there sulphate available in the medium?

is there a anom peak marking the S position - but you need good data to
hope to find this - you can verify quality by checking all other S in
the crystal

Eleanor

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: CCP4BB Digest - 9 Apr 2010 to 10 Apr 2010 (#2010-97)
From: Matthew E Thornton thornton {- at -} CALTECH {- dot -} EDU
Date: 2010-04-11

Next message:
Subject: Re: Phasing statistics
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2010-04-12