Re: [ccp4bb] Need isolated crystals

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: How to show electron density map for my ligand in pymol, which is bound in the active site..??/
From: Dirk Kostrewa kostrewa {- at -} GENZENTRUM {- dot -} LMU {- dot -} DE
Date: 2010-04-13

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Subject: Re: Need isolated crystals
From: Enrico Stura estura {- at -} CEA {- dot -} FR
Date: 2010-04-13

Subject: Re: Need isolated crystals
From: Tim Gruene tg {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2010-04-13

On Tue, Apr 13, 2010 at 09:24:13AM -0400, Nathaniel Clark wrote:
> [...] If you don't want to buy those tools just try 2 nylon loops, and lots
> of patience.
Or use cats whiskers which you can fix into a Pasteur pipette with wax, nail
varnish, plasticine, etc.

I find them to have just the right stiffness to work with crystals, the tip is
very pointy and does not fray.

You just need to know a cat owner and ask them to look out for the whiskers the
next time they vaccuum clean their flat.

Tim

> Nat
>
> On Tue, Apr 13, 2010 at 4:49 AM, Enrico Stura wrote:
> > Amit Sharma,
> >
> > I second Tim's suggestions.
> >
> > The first experiment is the following.
> > Set up several experiments at constantly decreasing precipitant
> > concentrations.
> > Streak seed all of them. If in any of those you can get non-clustered
> > crystals
> > non matter what size, seeding can solve your problem.
> > To restore crystal size follow the rest of Tim's list.
> >
> > Enrico.
> >
> >
> > On Mon, 12 Apr 2010 23:01:10 +0200, Tim Gruene
> > wrote:
> >
> >> Have you tried anything, yet? The list of answers can be quite long.
> >> A couple of keywords:
> >> - micro-seeding
> >> - macro-seeding
> >> - cross-seeding
> >> - additive screens
> >> - extend to purification protocol
> >> - change temperature, pH, etc.
> >> - have you searched around the current conditions
> >> ...
> >>
> >> if you describe a little what you have done so far we can also get an idea
> >> of
> >> your status of experience and adapt the answers accordingly.
> >>
> >> Kind regards, Tim
> >
> > --
> > Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office
> > Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab
> > LTMB, SIMOPRO, IBiTec-S, CE Saclay, 91191 Gif-sur-Yvette, FRANCE
> > http://www-dsv.cea.fr/en/institutes/institute-of-biology-and-technology-saclay-ibitec-s/unites-de-recherche/department-of-molecular-engineering-of-proteins-simopro/molecular-toxinology-and-biotechnology-laboratory-ltmb/crystallogenesis-e.-stura
> > http://www.chem.gla.ac.uk/protein/mirror/stura/index2.html
> > e-mail: estura@cea.fr Fax: 33 (0)1 69 08 90 71
> >

--
--
Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen

GPG Key ID = A46BEE1A

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Next message:
Subject: Re: Need isolated crystals
From: Enrico Stura estura {- at -} CEA {- dot -} FR
Date: 2010-04-13