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Re: [ccp4bb] Mysterious Crystals? |
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- Protein crystallographyMain steps:- Protein purification- Crystallisation Special:- Programs for crystallography- X-ray detectors Basic tutorials:- Chemistry- Protein - Peptide - Amino Acids Xtal community:- CCP4BB |
CCP4bb navigationCCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999Subject: Re: Mysterious Crystals? From: Jürgen_Bosch jubosch {- at -} JHSPH {- dot -} EDU Date: 2010-04-19 Fish and wash some crystals then run them on a SDS-gel, then you will know for sure if it's protein or not. Jürgen On Apr 18, 2010, at 10:46 PM, tat cheung cheng wrote: > Hi all, > > I have got some crystals, the purified protein was in Tris buffer with 300mM NaCl for crystallization. they grew in light weight PEG, PEG400 or monomethyl ethyl PEG500, they were needle shaped, could be long (~0.2mm) but very thin all the time and sometimes grew into sea-urchin like needle cluster. > What interesting is, when i gridded crystallization conditions against pH or PEG amount, the crystals sizes and shapes varied, and the crystals were fragile so i believed they were protein crystals in nature. But upon X-ray diffraction, they gave no reflection at all, not even a faint spot. > I wonder, beside silly mistakes like misalignment of the crystal to the beam, not enough exposure time, what could be the reason for this mysterious crystals? Are they protein or PEG or what? > Thanks very much. > > Tc > > > > - Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-3655 http://web.mac.com/bosch_lab/ CCP4bb navigationCCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999 |
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