Re: [ccp4bb] Rsym problems...maybe???

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: 3D system set up
From: lei feng spartanfenglei {- at -} HOTMAIL {- dot -} COM
Date: 2010-04-22

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Subject: Senior Director, Structural Biology (Takeda San Diego)
From: "Tatone, Josephine (TPNA)(Cont {- dot -} )" jtatone {- at -} TPNA {- dot -} COM
Date: 2010-04-22

Subject: Re: Rsym problems...maybe???
From: Jim Pflugrath jim {- dot -} pflugrath {- at -} RIGAKU {- dot -} COM
Date: 2010-04-22

Yeah, but how was I/sdI determined? Most programs allow you to multiply
your sdI by any number you want which in turns means that you can create
any I/sdI that you want.

A multiplicity of 11 does not explain a high Rsym to me.

Jim

On Thu, 22 Apr 2010, Frank von Delft wrote:

> Yeah, stop worrying! Your I/sdI is all that matters.
> phx
>
>
> On 22/04/2010 18:06, Daniel Bonsor wrote:
>> Hello again.
>>
>> At first I was not worry but maybe now I am. I have completed a structure
>> and submitted to the PDB. They queried my Rsym value in the highest
>> resolution bin, 2.5-2.37A (may I dare say it 100%). I was not worried at
>> the time as I had:
>>
>> 99.4% completeness
>> Mean(I/sdI) of 2.5
>> and a redundancy of 11 (which would explain the high Rsym)
>> Space group I422
>>
>> My Rpim in this shell is 30%.
>>
>> Should I reduce the resolution and start from scratch again or is
>> everything fine and dandy and I should stop worrying?
>>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: 3D system set up
From: lei feng spartanfenglei {- at -} HOTMAIL {- dot -} COM
Date: 2010-04-22

Next message:
Subject: Senior Director, Structural Biology (Takeda San Diego)
From: "Tatone, Josephine (TPNA)(Cont {- dot -} )" jtatone {- at -} TPNA {- dot -} COM
Date: 2010-04-22