Quick navigation: Home   |    Site Map   ||    References   |    Biography   ||    Copyright   |    Other copyright   |    Contact us   |    Advert   |   
 

Re: [ccp4bb] How to remove nucleic acid contamination for crystallizing zinc finger protein
- Protein crystallography

Main steps:

   - Protein purification
   - Crystallisation

Special:

   - Programs for crystallography
   - X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

   - CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Re: Off topic: where to order just miniprep columns?
From: Cynthia Kinsland clk10 {- at -} CORNELL {- dot -} EDU
Date: 2007-07-05		Next message:
Subject: Re: How to remove nucleic acid contamination for crystallizing zinc finger protein
From: Pavan p {- dot -} vaidyanathan {- at -} UMIAMI {- dot -} EDU
Date: 2007-07-05


Subject: Re: How to remove nucleic acid contamination for crystallizing zinc finger protein
From: Ana Silva silva {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-07-05

Hi,

How much salt do you have in your protein buffer?
I would try to increase the salt concetration, during purification.

Hope it helps.
Ana



tony_htc@126.com wrote:
>
> Dear all,
>
> Sorry for the off-topic question.
>
> I am purifying a zinc finger transcription factor for crystallization.
> The protein appeared as a single band on SDS-PAGE (MW 44KD) after NTA
> chelating column, but its OD280/OD260 ratio is as high as 1.0. So I
> doubt the protein is nucleic acid contaminated, probably because of
> the zinc finger. I tried to remove the nucleic acid by Mono Q and
> Superdex 75 pg, but failed. So could any one recommend some method to
> remove the nucleic acid during protein crystallization, esp. zinc
> finger protein? Any experience or references will be appreciated.
>
> Thanks a lot!
>
> Tiancen Hu
>
> Shanghai Institute of Materia Medica
>
>
>
> ------------------------------------------------------------------------
> Ò»Æð À´£¬150 Íò ÈË Í¬ ʱ ÔÚ Íæ µÄ ÃÎ »Ã Î÷ ÓÎ
>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Re: Off topic: where to order just miniprep columns?
From: Cynthia Kinsland clk10 {- at -} CORNELL {- dot -} EDU
Date: 2007-07-05		Next message:
Subject: Re: How to remove nucleic acid contamination for crystallizing zinc finger protein
From: Pavan p {- dot -} vaidyanathan {- at -} UMIAMI {- dot -} EDU
Date: 2007-07-05
ProteinCrystallography.org: Copyright 2006-2010 by Quid United Ltd