Re: [ccp4bb] How to remove nucleic acid contamination for crystallizing zinc finger protein

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: How to remove nucleic acid contamination for crystallizing zinc finger protein
From: Jeffrey {- dot -} Kieft {- at -} UCHSC {- dot -} EDU Jeffrey {- dot -} Kieft {- at -} UCHSC {- dot -} EDU
Date: 2007-07-05

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Subject: Re: How to remove nucleic acid contamination for crystallizing zinc finger protein
From: Raji Edayathumangalam redayath {- at -} MAIL {- dot -} ROCKEFELLER {- dot -} EDU
Date: 2007-07-05

Subject: Re: How to remove nucleic acid contamination for crystallizing zinc finger protein
From: M T michelthe {- at -} GMAIL {- dot -} COM
Date: 2007-07-05

Hi, I think that a good choice, if your protein accept, is to try a gel
filtration at high salt, with or without DNAse incubation before gel
filtration. If your FPLC can do it, a monitoring at 280 and 260 nm will help
you.

Michel

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