Re: [ccp4bb] Substitute for Lithium Sulfate

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Help with Bayes's theorem
From: Michel Fodje Michel {- dot -} Fodje {- at -} LIGHTSOURCE {- dot -} CA
Date: 2010-04-27

Next message:
Subject: Postdoctoral Fellowship in Membrane Protein Crystallography in Houston
From: "Spudich, John L" John {- dot -} L {- dot -} Spudich {- at -} UTH {- dot -} TMC {- dot -} EDU
Date: 2010-04-27

Subject: Re: Substitute for Lithium Sulfate
From: Ed Pozharski epozh001 {- at -} UMARYLAND {- dot -} EDU
Date: 2010-04-27

Transferring crystal into mother liquor minus lithium sulfate plus
ligand may work (consider both direct and stepwise transfer). Worked
for us recently when 0.2M sodium malonate was competing out the ligand
(which is ironic given that sodium malonate would be my first choice of
replacing ammonium sulfate when it is major precipitant, see Holyoak et
al., Acta Cryst D59:2356).

HTH,
Ed.

On Fri, 2010-04-23 at 12:53 -0500, Yi-Liang Liu wrote:
> Hi Everyone,
>
> I have a question about the crystallization condition. Currently the
> condition I use contains 0.2M lithium sulfate. However, the ligand
> there seems to compete the site where sulfate binds in the active
> site. Are there any good substitute to replace the Lithium sulfate in
> my crystallization buffer?
>
>
> Best,
>
>
> Lucas
>
>
>

--
Edwin Pozharski, PhD, Assistant Professor
University of Maryland, Baltimore
----------------------------------------------
When the Way is forgotten duty and justice appear;
Then knowledge and wisdom are born along with hypocrisy.
When harmonious relationships dissolve then respect and devotion arise;
When a nation falls to chaos then loyalty and patriotism are born.
------------------------------ / Lao Tse /

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Help with Bayes's theorem
From: Michel Fodje Michel {- dot -} Fodje {- at -} LIGHTSOURCE {- dot -} CA
Date: 2010-04-27