Re: [ccp4bb] Problems in MR

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CCP4bb <-- 2007 <-- March 2007 <-- 05 March 2007

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Subject: Re: SUMMARY: Cannot run NTA to purify the protein having Histag?
From: Tim Gruene tg {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2007-03-05

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Subject: Re: practical limits of MR?
From: William Scott wgscott {- at -} CHEMISTRY {- dot -} UCSC {- dot -} EDU
Date: 2007-03-05

Subject: Re: Problems in MR
From: Leo Chavas leo {- at -} PFWEIS {- dot -} KEK {- dot -} JP
Date: 2007-03-05

Dear Lei,

just in case: would that be possible that you are encountering a
swapping, ie 3D domain swapping, behavior? To check this, have a look
at the electron density around the "crash". If it is continuous, you
might consider this possibility.

Regards.
Leo

On Mar 3, 2007, at 12:10 AM, thunderbird wrote:

> Hi all.
> I am trying to solve a structure with one molecules in the asu
> through MR.
> Using a not good data set at 3.0A and a structure with sequence
> similarity of
> 37%, Phaser gave a result of z score 14.7 and LL-gain 220,
> probably a right
> solution.
> But when I slightly modified the model I got from Phaser and used
> it as an
> input to run Phaser again, I got another solution overlapped partly
> with the
> first solution. I don't know how it could happen like that. And
> the Rfree
> factor could not go
> below 0.43 in the following refinemet. I am wondering what is wrong
> with it?
> thanx in advance
> Lei Yin
>
>
> Lei Yin, Ph.D.
> National Laboratory of Biomacromolecules
> Institute of Biophysics
> Chinese Academy of Sciences
> No. 15 Datun Road, Beijing 100101, China
> E-mail:thunderbird@moon.ibp.ac.cn
> Tel: 86-10-64888548
>

==========================
Chavas Leonard M.G., Ph.D.
Structural Research Center
KEK,PF. 1-1 Oho
Tuskuba, Ibaraki - Japan
---------------------------------------------
PHS: +81(0)29-864-5200 (ext: 2682)
e-mail: leo@pfweis.kek.jp
URL: http://pfweis.kek.jp/~leo
==========================

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CCP4bb <-- 2007 <-- March 2007 <-- 05 March 2007

Previous message:
Subject: Re: SUMMARY: Cannot run NTA to purify the protein having Histag?
From: Tim Gruene tg {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2007-03-05

Next message:
Subject: Re: practical limits of MR?
From: William Scott wgscott {- at -} CHEMISTRY {- dot -} UCSC {- dot -} EDU
Date: 2007-03-05