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Re: [ccp4bb] control of nucleation
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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Re: control of nucleation
From: Tim Gruene tg {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2010-05-06		Next message:
Subject: Re: software to represent raw reflections in hkl zones
From: Nicholas M Glykos glykos {- at -} MBG {- dot -} DUTH {- dot -} GR
Date: 2010-05-06


Subject: Re: control of nucleation
From: Thomas Edwards T {- dot -} A {- dot -} Edwards {- at -} LEEDS {- dot -} AC {- dot -} UK
Date: 2010-05-06

Dear Zq,

A few ideas:

1) Vary protein concentration, temperature, or protein : mother liquor ratio.
2) Try dioxane - it is supposed to reduce nucleation.
3) give your protein a good hard spin before you set up drops to remove aggregates.
4) seeded factorial screen.
5) re-purify on gel filtration?

Ed

______________
T.Edwards Ph.D.
Garstang 8.53d
Astbury Centre for Structural Molecular Biology
University of Leeds, Leeds, LS2 9JT
Telephone: 0113 343 3031
http://www.bmb.leeds.ac.uk/staff/tae/
-- Nature composes some of her loveliest poems for the microscope and the telescope. ~Theodore Roszak



________________________________
From: zq deng
Reply-To: zq deng
Date: Thu, 6 May 2010 09:03:46 +0100
To:
Subject: [ccp4bb] control of nucleation

hello,everybody . due to excess nucleation,I often get many tiny crystals instead of few,large crystals.i wana optimize the condition, does anyone have adivce about this?

Best regards.
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