Quick navigation:        Home   |    Site Map   ||    References   |    Biography   ||    Copyright   |    Other copyright   |    Contact us   |   
Protein structure
 

Re: [ccp4bb] Solving structure of protein-protein complexes using MR
 

Basic tutorials: 		 
 

CCP4bb navigation

CCP4bb <-- 2007 <-- July 2007 <-- 06 July 2007
Previous message:
Subject: Solving structure of protein-protein complexes using MR
From: Joe Smith ewaldxray {- at -} GMAIL {- dot -} COM
Date: 2007-07-06 		Next message:
Subject: Re: The CCP4 license is ambiguous
From: "Herbert J {- dot -} Bernstein" yaya {- at -} BERNSTEIN-PLUS-SONS {- dot -} COM
Date: 2007-07-06


Subject: Re: Solving structure of protein-protein complexes using MR
From: "Manfred S {- dot -} Weiss" msweiss {- at -} EMBL-HAMBURG {- dot -} DE
Date: 2007-07-06
Hi Joe,

I think you are wasting your time pursueing molrep.

Go for some experimental phases. Try a bromide soak or xenon
derivatisation. Provided your molrep solution is correct,
you don't need a lot of extra phase information.

Cheers, Manfred.


********************************************************************
* *
* Dr. Manfred S. Weiss *
* *
* Team Leader *
* *
* EMBL Hamburg Outstation Fon: +49-40-89902-170 *
* c/o DESY, Notkestr. 85 Fax: +49-40-89902-149 *
* D-22603 Hamburg Email: msweiss@embl-hamburg.de *
* GERMANY Web: www.embl-hamburg.de/~msweiss/ *
* *
********************************************************************


On Fri, 6 Jul 2007, Joe Smith wrote:

> Hi all,
>
> We have been trying to solve a structure of protein-protein complexes using
> 3.1A data (one of the proteins is of 120kDa whereas the other is of 20kDa).
> The structure of smaller protein is known (individually-100% sequence
> identity) whereas the bigger protein does not share more than 10% sequence
> identity with the similar proteins solved from other sources.
>
> Due to some problem in getting Seleno-labelled protein, we have also been
> trying to use molecular replacement (MR) to solve the structure. We want to
> find out the correct position of smaller protein using MR and then plan to
> extend the phases to the whole asymmetric unit (we hope it could be done but
> not sure). We are more or less sure about the fold of bigger protein and
> expect it to be similar to the other known related structures.
> In one of the solution obtained using phaser, the map looks really good, but
> this solution doesn't provide good packing of the complex inside the unit
> cell. Due to low scattering contribution of the smaller protein, we are
> unable to refine any possible solutions using REFMAC.
>
> We welcome any kind of suggestions in this regard.
>
> Thanking you in advance.
>
> Joe
>

CCP4bb navigation

CCP4bb <-- 2007 <-- July 2007 <-- 06 July 2007
Previous message:
Subject: Solving structure of protein-protein complexes using MR
From: Joe Smith ewaldxray {- at -} GMAIL {- dot -} COM
Date: 2007-07-06 		Next message:
Subject: Re: The CCP4 license is ambiguous
From: "Herbert J {- dot -} Bernstein" yaya {- at -} BERNSTEIN-PLUS-SONS {- dot -} COM
Date: 2007-07-06


ProteinCrystallography.org: Copyright 2006-2007 by Quid United Ltd