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Re: [ccp4bb] thread protein sequence using pdb coordinates that are not avaliable on RCSB? |
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- Protein crystallographyMain steps:- Protein purification- Crystallisation Special:- Programs for crystallography- X-ray detectors Basic tutorials:- Chemistry- Protein - Peptide - Amino Acids Xtal community:- CCP4BB |
CCP4bb navigationCCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999Subject: Re: thread protein sequence using pdb coordinates that are not avaliable on RCSB? From: Scott Pegan scott {- dot -} d {- dot -} pegan {- at -} GMAIL {- dot -} COM Date: 2010-05-12 Kelly, You can do it with swiss-model, just have to use the alignment mode. I will ask for a pdb or RCSB code. Also, in the past I have found Jigsaw 3D model server to be a comparable tool. Scott On Wed, May 12, 2010 at 10:10 AM, Kelly Daughtry > Hello all, > I would like to thread one protein sequence onto a structure I recently > solved, and has not been submitted to the pdb. > > I found the swiss-model website, which is an excellent tool for inputing a > template sequence and target sequence (which is what I want to do), but it > only allows you to select a PDB ID, and not to upload your own pdb file. > > Does anyone know where I can do this online? I'm thisclose to just changing > each amino acid in coot (with the amount of searching I've done, it's about > equal time!). > > Thanks in advance, > Kelly Daughtry > > ******************************************************* > Kelly Daughtry > PhD Candidate > Department of Physiology and Biophysics > Boston University School of Medicine > 590 Commonwealth Ave > R 390 > Boston MA, 02215 > (P) 617-358-5548 > ******************************************************* > -- Scott D. Pegan, Ph.D. Assistant Professor Chemistry & Biochemistry University of Denver CCP4bb navigationCCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999 |
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