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Re: [ccp4bb] About seeding in microbatch under oil crystallization
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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
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Subject: Re: Should I be worried about negative electron density?
From: Clemens Vonrhein vonrhein {- at -} GLOBALPHASING {- dot -} COM
Date: 2010-05-19		Next message:
Subject: Re: Should I be worried about negative electron density?
From: Thomas Womack twomack {- at -} GLOBALPHASING {- dot -} COM
Date: 2010-05-19


Subject: Re: About seeding in microbatch under oil crystallization
From: Harm Otten harm82 {- at -} GMAIL {- dot -} COM
Date: 2010-05-19

Hello Donghui
we used a robot to setup the batch crystallization screens with some
microseed stock solution and the relevant protein and reservoir
stocks. That worked fine. I only mention a collegues work here, so if
there are more detailed questions, I will approach him.
Good luck,

Harm

On Wed, May 19, 2010 at 11:28 AM, wu donghui wrote:
> Hi ccp4bbers,
>
> I want to know if anyone has any experience about seeding (streak seeding or
> microseeding) in microbatch under oil crystallization. I wonder if the oil
> might block or wipe away the seeds if cat whisker is used for streak
> seeding. Thank you for your input ahead.
>
> Best regards,
>
> Donghui



--
Harm Otten
Department of Chemistry
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2100 Copenhagen Ø
Denmark
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email harm@kemi.ku.dk
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