Re: [ccp4bb] insect cell media

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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From: rashmi panigrahi rashmi {- dot -} panigrahi19 {- at -} GMAIL {- dot -} COM
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Date: 2010-06-07

Subject: Re: insect cell media
From: Chris Ulens chris {- dot -} ulens {- at -} MED {- dot -} KULEUVEN {- dot -} BE
Date: 2010-06-07

Hi,
I would like to get some feedback on the type of media that people are
using for insect cell culturing.
We have happily made a transition last year from using Invitrogen's
SFM medium and cellfectin to Insect-Xpress (Lonza) and
polyethyleneimine for transfection. We are moving several protein
targets to large-scale cultures and would consider cost-cutting
alternatives. For example, Invitrogen and Thermo Sci both offer media
in powder form at an attractive price. Has anyone made a systematic
comparison of these media? Any particular recommendations regarding
powder media?

Thanks.
-Chris

---------------------------------------------------
Chris Ulens, Ph.D.
Lab of Structural Neurobiology
Department of Molecular Cell Biology
Campus Gasthuisberg, ON1
Herestraat 49, PB 601
B-3000 Leuven
Belgium
e chris.ulens@med.kuleuven.be
t +32 16 345812
f +32 16 345699
w http://www.xtal.be

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: dissolving peptides !
From: rashmi panigrahi rashmi {- dot -} panigrahi19 {- at -} GMAIL {- dot -} COM
Date: 2010-06-07

Next message:
Subject: Re: Fwd: creating a metal plane model to fit a protein.
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2010-06-07