Re: [ccp4bb] dissolving peptides !

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

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   - Amino Acids

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: insect cell media
From: Tommi Kajander tommi {- dot -} kajander {- at -} HELSINKI {- dot -} FI
Date: 2010-06-07

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From: A Leslie andrew {- at -} MRC-LMB {- dot -} CAM {- dot -} AC {- dot -} UK
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Subject: Re: dissolving peptides !
From: gauri misra kamgauri {- at -} GMAIL {- dot -} COM
Date: 2010-06-07

Rashmi,
You can try 1%DMSO and also acetonitrile but the former is preferable.

Gauri

On Mon, Jun 7, 2010 at 3:04 AM, rashmi panigrahi <
rashmi.panigrahi19@gmail.com> wrote:

> Hello everybody,
> I have a 16 mer peptide which is predicted to be positively charged alpha
> helix and has 50% of its sequence hydrophobic.
>
> Could any one recommend the best way to dissolve it, and then it can be
> used for crystallization trials.
>
> thanks
>
> --
> rashmi
>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: insect cell media
From: Tommi Kajander tommi {- dot -} kajander {- at -} HELSINKI {- dot -} FI
Date: 2010-06-07

Next message:
Subject: Re: Scaling question
From: A Leslie andrew {- at -} MRC-LMB {- dot -} CAM {- dot -} AC {- dot -} UK
Date: 2010-06-07