Re: [ccp4bb] practical limits of MR?

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: MTZ format conversion
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-03-06

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From: Kevin Cowtan cowtan {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-03-06

Subject: Re: practical limits of MR?
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-03-06

Congratulations - That is amazing - did you find both molecules even
though they differ?
Eleanor
William Scott wrote:
> We just solved a 142 nucleotide asymmetric unit of a novel
> ribozyme structure using only A-form RNA helical fragments and phaser. I'm
> trying to find some time to write the paper but the basic idea is sketched
> out in the supplementary material to the paper that comes out March 16th
> in Science.
>
> I was fairly stunned that it worked. I was trying to get a low-res mask
> to aid in finding 22 Br atoms (which we never used, except to confirm the
> trace after the fact). Although there were 2 molecules in the asymmetric
> unit, they differ radically and no 2-fold symmetry could be used to help.
>
> Bill
>
>
> On Mon, 5 Mar 2007, Nat Echols wrote:
>
> I had a debate with a coworker about using MR in desperation and I'm
> curious what the most extreme case is where a very different model was used
> to solve a structure. This could be highest RMSD, lowest % identity, or
> most incomplete model. I'm also curious whether homology modelling has
> ever been useful for this. (I'm pretty sure I've come across papers
> discussing this last concept.)
>
> thanks,
> Nat
>
>
>
>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: MTZ format conversion
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-03-06

Next message:
Subject: Re: MTZ format conversion
From: Kevin Cowtan cowtan {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-03-06