Re: [ccp4bb] Help with reducing crystal mosaicity

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

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- X-ray detectors

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: Help with reducing crystal mosaicity
From: Tim Grune tim {- dot -} grune {- at -} SYNCHROTRON {- dot -} VIC {- dot -} GOV {- dot -} AU
Date: 2007-07-09

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Subject: Re: anisotropic displacement parameter matrix shelxl
From: "Ralf W {- dot -} Grosse-Kunstleve" rwgk {- at -} YAHOO {- dot -} COM
Date: 2007-07-09

Subject: Re: Help with reducing crystal mosaicity
From: Juergen Bosch jbosch {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2007-07-09

Hi Mary,

not sure if you received this suggestion already.
Despite the low resolution diffraction 4-5 A as you report, try mounting
smaller crystals and see how well they perform. Very often our crystals
had a better freezing experience when they were small, even if
sufficient cryo protectant was present.

Another thing to try is once you have mounted a crystal, how does it
behave after flash annealing / wet annealing / multiple rounds of
annealing ?

Juergen

--
Jürgen Bosch
University of Washington
Dept. of Biochemistry, K-426
1705 NE Pacific Street
Seattle, WA 98195
Box 357742
Phone: +1-206-616-4510
FAX: +1-206-685-7002

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Help with reducing crystal mosaicity
From: Tim Grune tim {- dot -} grune {- at -} SYNCHROTRON {- dot -} VIC {- dot -} GOV {- dot -} AU
Date: 2007-07-09

Next message:
Subject: Re: anisotropic displacement parameter matrix shelxl
From: "Ralf W {- dot -} Grosse-Kunstleve" rwgk {- at -} YAHOO {- dot -} COM
Date: 2007-07-09