Re: [ccp4bb] Help with reducing crystal mosaicity

- Protein crystallography

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Subject: Re: Help with reducing crystal mosaicity
From: Marius Schmidt marius {- dot -} schmidt {- at -} PH {- dot -} TUM {- dot -} DE
Date: 2007-07-11

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Subject: Re: program to create cartoon image from a PDB file which have only C-alpha coordinates
From: Tim Grune tim {- dot -} grune {- at -} SYNCHROTRON {- dot -} VIC {- dot -} GOV {- dot -} AU
Date: 2007-07-11

Subject: Re: Help with reducing crystal mosaicity
From: "Moody, Dr P {- dot -} C {- dot -} E {- dot -} " pcem1 {- at -} LEICESTER {- dot -} AC {- dot -} UK
Date: 2007-07-11

a couple of points from long BC about approx. 0 C cooling...for what its worth!

i) don't forget that high concentrations of salts can have a significant effect on the freezing point ( remember freezing point depression?). We used to collect data at -15C from crystals grown in 2.4M Ammonium Sulphate

ii) cool slowly and gently over a couple of hours

iii) put the mother liquor at only the colder end of the capillary to prevent condensation

iv) we found a transition at about -5 for above crystals, so be careful.

Peter Moody
Henry Wellcome Laboratories of Structural Biology
Department of Biochemistry
Henry Wellcome Building
University of Leicester
LE1 9HN
0116 229 7097

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From: CCP4 bulletin board on behalf of mesters
Sent: Wed 11/07/2007 08:46
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Help with reducing crystal mosaicity

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Help with reducing crystal mosaicity
From: Marius Schmidt marius {- dot -} schmidt {- at -} PH {- dot -} TUM {- dot -} DE
Date: 2007-07-11