Re: [ccp4bb] monomeric coiled coil

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: monomeric coiled coil--updated
From: chern chern {- at -} UALBERTA {- dot -} CA
Date: 2010-07-04

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Subject: OFF: Intein-chitin purification method
From: Petr Kolenko kolenko {- at -} IMC {- dot -} CAS {- dot -} CZ
Date: 2010-07-05

Subject: Re: monomeric coiled coil
From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2010-07-04

> 4. The physiological concentration is a bit misleading. First, its clear
> now that cells have microenvironments,
> and 'physiological' concentrations are hard to define. Also, in a cell, I
> think (and I think others tend to agree)
> that kD plays little role at the end. kD is a combination of k(on) -
> which is concentration dependent but in a cell
> very likely diffusion limited - and of k(off) which I think is what
> matters most in the cell.
>

Can you provide some references about kD not mattering in cells? I had
thought it a basic tenet that kD was a major indication of physiological
relevence. After all, one could presumably determine a kD for any two
proteins, so the order of magnitude of the kD would seem to matter. I hear
that your argument is more subtle in considering the elements of kD, but
isn't kD usually a rough indicator of k(off), and of course more
readily-accessible experimentally? I am intrigued by the idea...

Jacob Keller

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: monomeric coiled coil--updated
From: chern chern {- at -} UALBERTA {- dot -} CA
Date: 2010-07-04

Next message:
Subject: OFF: Intein-chitin purification method
From: Petr Kolenko kolenko {- at -} IMC {- dot -} CAS {- dot -} CZ
Date: 2010-07-05