Re: [ccp4bb] Easy proteins to crystallize

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: COOT question
From: "Meier Chris (SLH)" Chris {- dot -} Meier {- at -} UCB-GROUP {- dot -} COM
Date: 2007-03-07

Next message:
Subject: Re: Very weird
From: Edwin Pozharski epozh001 {- at -} UMARYLAND {- dot -} EDU
Date: 2007-03-07

Subject: Re: Easy proteins to crystallize
From: "Douglas L {- dot -} Theobald" dtheobald {- at -} BRANDEIS {- dot -} EDU
Date: 2007-03-07

Thanks to all who have responded. Any last minute suggestions before
I summarize?

On Mar 1, 2007, at 7:40 PM, mjvdwoerd@netscape.net wrote:

> I really like to use glucose isomerase, for sale from for example
> Hampton. It is a crystalline suspension when you buy it and it is
> trivial to get crystals with almost any precipitant - we have
> managed to crystallize it from water with temperature
> variation :-). Some precipitants give multiple crystal habits (and
> spacegroups). There is a clear correlation between recipe and
> quality. For example, you can grow beautiful crystals with ammonium
> sulfate but those are next-to-impossibe to cryo-cool, while others
> are much easier.
> Protein is a lot bigger than lysozyme and insulin, so perhaps more
> representative of a 'typical protein'. The crystals diffract like
> rocks.
>
> When I get to setting up a class to teach, I think that is what I
> will use.
>
> Mark
>
> Mark van der Woerd, PhD
> Research Scientist
> Dept. of Biochemistry and Molecular Biology
> Colorado State University
> Fort Collins, CO 80523
> Phone (970) 491-0469
>
> -----Original Message-----
> From: dtheobald@BRANDEIS.EDU
> To: CCP4BB@JISCMAIL.AC.UK
> Sent: Thu, 22 Feb 2007 11:23 AM
> Subject: [ccp4bb] Easy proteins to crystallize
>
> Hi all,
>
> I'd like to pick the collective brain of crystallographers on this
> list -- what are some of the most easily crystallizable proteins?
> I'm especially interested in those that over-express and diffract
> well, and in ones that might be less well-known than, say, lysozyme
> (but nearly as nice).
>
> Douglas
>
>
> ^`^`^`^`^`^`^`^`^`^`^`^`^`^`^`^`^`^`^`^`
> Douglas L. Theobald
> Department of Biochemistry
> Brandeis University
> Waltham, MA 02454-9110
>
> dtheobald@brandeis.edu
>
> ^
> /` /^. / /
> / / /`/ / . /`
> / / ' '
> '
> Check Out the new free AIM(R) Mail -- 2 GB of storage and industry-
> leading spam and email virus protection.

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: COOT question
From: "Meier Chris (SLH)" Chris {- dot -} Meier {- at -} UCB-GROUP {- dot -} COM
Date: 2007-03-07

Next message:
Subject: Re: Very weird
From: Edwin Pozharski epozh001 {- at -} UMARYLAND {- dot -} EDU
Date: 2007-03-07