Re: [ccp4bb] Mico-focus beamline

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Mico-focus beamline
From: "David J {- dot -} Schuller" djs63 {- at -} CORNELL {- dot -} EDU
Date: 2007-07-20

Next message:
Subject: Re: Mico-focus beamline
From: "Sanishvili, Ruslan" rsanishvili {- at -} ANL {- dot -} GOV
Date: 2007-07-20

Subject: Re: Mico-focus beamline
From: Nazzareno Dimasi ndimasi {- at -} GMAIL {- dot -} COM
Date: 2007-07-20

Hi Peng,

I am not sure where to go in the US, but the microfocus beamline ID23-2
at the ESRF is the place that very well fit your case (Please see the two
publications below)!

Regards,

Nazzareno.

1: Dimasi N, Flot D, Dupeux F, Marquez JA. Expression, crystallization
and X-ray data collection from microcrystals of the extracellular domain
of the human inhibitory receptor expressed on myeloid cells
IREM-1. Acta Crystallograph Sect F Struct Biol Cryst Commun.
2007 Mar 1;63(Pt 3):204-8.

2: Marquez JA, Galfre E, Dupeux F, Flot D, Moran O, Dimasi N. The crystal
structure of the extracellular domain of the inhibitor receptor
expressed on myeloid cells IREM-1. J Mol Biol. 2007 Mar 23;367(2):310-8.
On 7/20/07, Peng Xie wrote:
>
> Hi, all
>
> I am working with this protein that only gives very thin needle crystals
> (25micron by 25 micron by 100-200micron) despite of various atempts to make
> it bigger, I am wondering if anybody here has any information as to where I
> can find a micro-focus beamline that will work on tiny needle crystals of
> this size in US.
>
> Also, I tried without any luck to do microseeding with my crystals using
> microbatch method which is the only method that will give me crystals, it
> has been a big concern that the tiny seed crystals I collected on the hair
> for streak seeding will be wiped out when I drag the hair through a layer of
> oil used for microbatch to seed new drops. It will be great if anybody has
> some hands on experience on doing microseeding in microbatch can give me
> some insights on this problem.
>
> Thanks you very much in advance for all your help!
>
> Best,
>
> Peng
>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Mico-focus beamline
From: "David J {- dot -} Schuller" djs63 {- at -} CORNELL {- dot -} EDU
Date: 2007-07-20

Next message:
Subject: Re: Mico-focus beamline
From: "Sanishvili, Ruslan" rsanishvili {- at -} ANL {- dot -} GOV
Date: 2007-07-20