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Re: [ccp4bb] speeding crystallisation
 

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CCP4bb <-- 2007 <-- August 2007 <-- 06 August 2007
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Subject: Re: speeding crystallisation
From: Zhen Zhang zz2121 {- at -} COLUMBIA {- dot -} EDU
Date: 2007-08-06
Hi Neeraj,

Two more tricks:
1. Open the cover slip a bit to allow for faster evaporation. It
worked well on one of my proteins.
2. in-situ proteolysis: Acta Crystallograph Sect F Struct Biol Cryst
Commun. 2006 Oct 1;62(Pt 10):1041-5.

Good luck.

Zhen

Quoting Neeraj :

> hi all,
> I am working on a protein for which we get nicely
> diffracting
> crystals but the problem is that the crystals grow from anywhere
> between
> 4-6 months. Does anyone has any general suggestions as to what
> things
> can be changed or tried to speed up the process. Any help or
> suggestions
> would be welcome as i am crawling towards getting enough crystals
> to
> make a synchroton visit:):)
>
> thanks
> Neeraj
>
> --
> Neeraj Kapoor
> TPCB Graduate Fellow
> Sakmar Lab/ Molecular Biology & Biochemistry
> The Rockefeller University
> 1230 York Avenue, RRB 510
> New York, NY 10021
> lab.1.212.327.8284:fax.7904
> mobile: 917.535.2030
> http://www.rockefeller.edu/labheads/sakmar/sakmar-lab.html
>


--
Zhen Zhang
Postdoctoral Associate
Howard Hughes Medical Institute
Department of Biochemistry and Molecular Biophysics
Columbia University
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