Re: [ccp4bb] needles vs crystals

- Protein crystallography

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: needles vs crystals
From: mjvdwoerd {- at -} NETSCAPE {- dot -} NET mjvdwoerd {- at -} NETSCAPE {- dot -} NET
Date: 2007-08-07

Shivesh,

In addition to the comments that have been made already, I would try seeding with the needles (or pieces thereof) - macroscopic, or take a couple of needles and crush them up into really small pieces and use various dilutions of these very small pieces for seeding. You could do that by serial dilution and adding a small amount to each new drop, or you could use the cat whisker /horse hair approach to distribute some of the nuclei to new drops.

Good luck.

Mark

-----Original Message-----
From: shivesh kumar <2shivesh@GMAIL.COM>
To: CCP4BB@JISCMAIL.AC.UK
Sent: Tue, 7 Aug 2007 8:45 am
Subject: [ccp4bb] needles vs crystals

Dear all,

I am trying to crystallize a 7kDa protein with MPD as a precipitant.I got the needles with precipitation in two days at 30% and in 4-5 days,needles appear in all the drops(pH 3.8-4.8).The concentration of protein is around 20mg/ml and the volume of mother liquor in
500microlt.What should I do to get good crystals,the temp. I am trying is 16C.Thanx in advance for the suggestions.

Shivesh Kumar

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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