[ccp4bb] Removal of bacterial Hsp70 contaminant from recombinant protein

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: ANIS and SHELXL
From: "George M {- dot -} Sheldrick" gsheldr {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2007-03-09

Next message:
Subject: Re: ANIS and SHELXL
From: Artem Lyubimov lyubimov {- at -} CALMAIL {- dot -} BERKELEY {- dot -} EDU
Date: 2007-03-09

Subject: Removal of bacterial Hsp70 contaminant from recombinant protein
From: Yong Tang liutang19 {- at -} GMAIL {- dot -} COM
Date: 2007-03-09

RE: Removal of bacterial chaperone Hsp70 contaminant from recombinant
protein preparation

Dear all,

I have a protein expressed at 37C for 3 hours in BL21 DE3 and purified
with sub-stoichiometric amount of apparent Hsp70 contaminant even
after exhaustive affinity (GST-fusion or His-tagged), ion-exchange and
sizing column steps. I would like to know if you have a
well-established protocol for getting rid of such a contaminant. I
asked around and was told to try adding ATP at certain(?) stage of the
purification.

I would much appreciate your input.

Many thanks! –yong @ the Wistar Institute

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: ANIS and SHELXL
From: "George M {- dot -} Sheldrick" gsheldr {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2007-03-09

Next message:
Subject: Re: ANIS and SHELXL
From: Artem Lyubimov lyubimov {- at -} CALMAIL {- dot -} BERKELEY {- dot -} EDU
Date: 2007-03-09