[ccp4bb] domains missing

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: CCP4 rotation convention
From: Ian Tickle I {- dot -} Tickle {- at -} ASTEX-THERAPEUTICS {- dot -} COM
Date: 2007-08-13

Next message:
Subject: domains missing
From: "Wu, Mousheng" Mousheng {- dot -} Wu {- at -} UTH {- dot -} TMC {- dot -} EDU
Date: 2007-08-13

Subject: domains missing
From: "Wu, Mousheng" Mousheng {- dot -} Wu {- at -} UTH {- dot -} TMC {- dot -} EDU
Date: 2007-08-13

Hi, ccp4 community,

I am solving my protein (300 aa) structure using molecular replacement.
The space group is P622. There is only one molecule in the ASU. The
protein is supported to have three domains. We have solved the domain 1
(120aa) structure; therefore we tried to use it as a model to solve the
new structure. MolRep and Phaser can find the domain 1. We refined the
model and the R and Rfree factor was around 50%. When we used Coot to
see the model and map, we found that the other two domains are missing.
In the map, there is a big 'hole' and little extra density (with 6-fold)
inside. It is a big surprise to us. Is there any possibility that R is
around 50 if the other two domains are missing?

Any suggests to figure out what happen to my structure?

Thanks a lot!

Mousheng Wu

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: CCP4 rotation convention
From: Ian Tickle I {- dot -} Tickle {- at -} ASTEX-THERAPEUTICS {- dot -} COM
Date: 2007-08-13

Next message:
Subject: domains missing
From: "Wu, Mousheng" Mousheng {- dot -} Wu {- at -} UTH {- dot -} TMC {- dot -} EDU
Date: 2007-08-13