[ccp4bb] extra density on Cysteine

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: extra density on Cysteine
From: xu {- dot -} ting {- at -} NOVARTIS {- dot -} COM xu {- dot -} ting {- at -} NOVARTIS {- dot -} COM
Date: 2007-08-13

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Subject: Re: extra density on Cysteine
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2007-08-13

Subject: extra density on Cysteine
From: xu {- dot -} ting {- at -} NOVARTIS {- dot -} COM xu {- dot -} ting {- at -} NOVARTIS {- dot -} COM
Date: 2007-08-13

Dear all,

I am refining a 2.0A structure. I found that there were some extra density
on two cysteines, even though I have added 5mM BME in the protein buffer.

I am wondering whether the first one (Cys292) is a bme and the second one
is an oxidized cysteine. Any suggestion?

I attached the images for your reference. thanks

Regards
_________________________________________
Xu Ting ,Ph.D
10 Biopolis Road
Singapore 138670
Fax: +65 6722 2916
Phone: +65 6722 2980

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: extra density on Cysteine
From: xu {- dot -} ting {- at -} NOVARTIS {- dot -} COM xu {- dot -} ting {- at -} NOVARTIS {- dot -} COM
Date: 2007-08-13

Next message:
Subject: Re: extra density on Cysteine
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2007-08-13