Re: [ccp4bb] extra density on Cysteine

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: coot in stereo
From: "Schubert, Carsten [PRDUS]" CSCHUBER {- at -} PRDUS {- dot -} JNJ {- dot -} COM
Date: 2007-08-14

Next message:
Subject: Re: CCP4 rotation convention
From: Ian Tickle I {- dot -} Tickle {- at -} ASTEX-THERAPEUTICS {- dot -} COM
Date: 2007-08-14

Subject: Re: extra density on Cysteine
From: Stephen Graham stepheng {- at -} STRUBI {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2007-08-14

2cystein.jpeg looks just like oxidation of cysteine to
S-hydroxy-cysteine (a.k.a. cysteine sulfenic acid). I have seen this
repeatedly in one of my structures (E. coli aminopeptidase P, see 1WL9
for an example). We discuss this a bit in Graham et al (2005)
Biochemistry 44: 13820-36 - see Figure 2 and surrounding text.

Cheers,

Stephen

On 8/14/07, xu.ting@novartis.com wrote:
>
> Dear all,
>
> I am refining a 2.0A structure. I found that there were some extra density
> on two cysteines, even though I have added 5mM BME in the protein buffer.
>
> I am wondering whether the first one (Cys292) is a bme and the second one is
> an oxidized cysteine. Any suggestion?
>
> I attached the images for your reference. thanks
>
> Regards
> _________________________________________
> Xu Ting ,Ph.D
> 10 Biopolis Road
> Singapore 138670
> Fax: +65 6722 2916
> Phone: +65 6722 2980
>

--
Dr Stephen Graham
Nuffield Medical Fellow
Division of Structural Biology
Wellcome Trust Centre for Human Genetics
Roosevelt Drive
Oxford OX3 7BN
United Kingdom
Phone: +44 1865 287 549

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: coot in stereo
From: "Schubert, Carsten [PRDUS]" CSCHUBER {- at -} PRDUS {- dot -} JNJ {- dot -} COM
Date: 2007-08-14

Next message:
Subject: Re: CCP4 rotation convention
From: Ian Tickle I {- dot -} Tickle {- at -} ASTEX-THERAPEUTICS {- dot -} COM
Date: 2007-08-14