[ccp4bb] modelling with sad/mad data

- Protein crystallography

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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From: lieven {- dot -} buts {- at -} VUB {- dot -} AC {- dot -} BE lieven {- dot -} buts {- at -} VUB {- dot -} AC {- dot -} BE
Date: 2007-03-10

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Date: 2007-03-12

Subject: modelling with sad/mad data
From: Peng Zhang pzhang {- at -} SIBS {- dot -} AC {- dot -} CN
Date: 2007-03-11

Dear friends,

Recently, I have solved a structure using mad method. When using the peak
data(2.3A) as the native for structure refinement, the gap between R
factor and R free is big, about 0.1(0.22 and 0.32). I modelled the
selenomethionine but the gap still exists. When I changed the data for a
real native one(2.7A),it seems OK with R=0.24 and Rf=0.28.

Does anyone have the similar experience?
what should I pay attention to when using the sad/mad data as the native
one for modelling and refinement?

Thanks in advance.

--
Peng Zhang, Ph.D. Student
Institute of Biochemistry and Cell Biology
Shanghai Institutes for Biological Sciences
Chinese Academy of Sciences

320 Yue-Yang Road
Shanghai 20031
P.R. China

Tel: 021-5492-1117
Fax: 021-5492-1116
Email: pzhang@sibs.ac.cn

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: CNS map and Coot
From: lieven {- dot -} buts {- at -} VUB {- dot -} AC {- dot -} BE lieven {- dot -} buts {- at -} VUB {- dot -} AC {- dot -} BE
Date: 2007-03-10

Next message:
Subject: Phaser TFZ=100.0 ?
From: Andrew Wong 6ahyw {- at -} QLINK {- dot -} QUEENSU {- dot -} CA
Date: 2007-03-12