Re: [ccp4bb] crystal with precipitation

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

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- X-ray detectors

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: ccp4 pack images program?
From: "Winter, G (Graeme)" g {- dot -} winter {- at -} DL {- dot -} AC {- dot -} UK
Date: 2007-08-23

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Subject: Re: crystal with precipitation
From: James Whisstock James {- dot -} Whisstock {- at -} MED {- dot -} MONASH {- dot -} EDU {- dot -} AU
Date: 2007-08-23

Subject: Re: crystal with precipitation
From: shivesh kumar 2shivesh {- at -} GMAIL {- dot -} COM
Date: 2007-08-23

Dear all
I welcome all the suggestions regarding my crystals which is coming with the
precipitation.The pI of the protein is 4.2 and the drop precipitates with
MPD as low as 30% within 4-5 hrs.I am trying the pH ranging from 3.6-5.2 at
16C.There is no precipitation at 20% of MPD.Also,at high conc,the protein
precipitates.The crystals are coming as florets as I mentioned with
2+2.Should I go for higher pH like uptp 8.5 or so.crystals are not growing
bigger in size so that I can mount.Should I try changing the
temperature.Ican use 4C and
16C.Right now I am keeping plates at 16C.I am concentrating the protein
through lyophilization.As soon I pool it for Gel filtration,majority of it
get precipitated,not soluble protein.Is there any method to make the protein
stable?
I appreciate all the informative suggestions.Thanx in advance and thanx
everybody who has suggested the various options.
Shivesh kumar

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: ccp4 pack images program?
From: "Winter, G (Graeme)" g {- dot -} winter {- at -} DL {- dot -} AC {- dot -} UK
Date: 2007-08-23

Next message:
Subject: Re: crystal with precipitation
From: James Whisstock James {- dot -} Whisstock {- at -} MED {- dot -} MONASH {- dot -} EDU {- dot -} AU
Date: 2007-08-23