Quick navigation: Home   |    Site Map   ||    References   |    Biography   ||    Copyright   |    Other copyright   |    Contact us   |    Advert   |   
 

Re: [ccp4bb] [off topic] Control of crystals' direction and position in the drop.

- Protein crystallography

Main steps:

   - Protein purification
   - Crystallisation

Special:

   - Programs for crystallography
   - X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

   - CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Membrane Protein Chemist-Staff Scientist opening at Takeda Pharmaceuticals (San Diego)
From: "Royer, Rick (TPNA)(Cont {- dot -} )" rick {- dot -} royer {- at -} TAKEDA {- dot -} COM
Date: 2011-11-14
Next message:
Subject: Error in overlapmap
From: Rojan Shrestha rojan {- at -} RIKEN {- dot -} JP
Date: 2011-11-15


Subject: Re: [off topic] Control of crystals' direction and position in the drop.
From: Frederic VELLIEUX frederic {- dot -} vellieux {- at -} ORANGE {- dot -} FR
Date: 2011-11-14

Hi,

Your email mentions "drop".

What about trying another technique where you do not have "drops", such as the liquid interface diffusion method (in capillaries), or the use of dialysis ? Crystallisation
under oil (injection of the 2 components under "oil") could also be tried.

Fred

> Message du 14/11/11 22:15
> De : "Nian Huang"
> A : CCP4BB@JISCMAIL.AC.UK
> Copie à :
> Objet : [ccp4bb] [off topic] Control of crystals' direction and position in the drop.
>
> Dear All,
> Does anybody find a way to control a crystal's positioning in the drop? I
> have needle shaped crystals. What I found out is that the vertical
> positioned crystals always grow much thicker than the crystals laying
> flatly. But unfortunately, it is a completely random event and only 1%
> crystals can appear vertically. I have tried different formats of plates
> and equilibration techniques w/o much success. Any suggestion is highly
> appreciated.
>
> Nian Huang, Ph.D.
> UT Southwestern Medical Center
>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Membrane Protein Chemist-Staff Scientist opening at Takeda Pharmaceuticals (San Diego)
From: "Royer, Rick (TPNA)(Cont {- dot -} )" rick {- dot -} royer {- at -} TAKEDA {- dot -} COM
Date: 2011-11-14
Next message:
Subject: Error in overlapmap
From: Rojan Shrestha rojan {- at -} RIKEN {- dot -} JP
Date: 2011-11-15



ProteinCrystallography.org: Copyright 2006-2010 by Quid United Ltd