[ccp4bb] solving structure of which 70% is known

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: exporting the "NCS found for Chain ... onto Chain ..." map
From: J {- dot -} Sanchez-weatherby {- at -} UEA {- dot -} AC {- dot -} UK J {- dot -} Sanchez-weatherby {- at -} UEA {- dot -} AC {- dot -} UK
Date: 2007-08-29

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Subject: Question about the percentages of occupancy for dual conformations.
From: Jian Wu protease {- at -} GMAIL {- dot -} COM
Date: 2007-08-30

Subject: solving structure of which 70% is known
From: Raja Dey rdey {- at -} USC {- dot -} EDU
Date: 2007-08-29

Hello,
I am trying to solve a multi-protein DNA complex structure from a 3.6 A native data set. The target structure is a dimer (95 aa in each monomer) in complex with DNA( 15 base pairs) plus a second protein of 131 aa. The data has been scaled to P6(1)22 sp. gr. and one target structure is expected to be in the asymmetric unit that corresponds to 68% solvent content. 70 % of the target structure is known in two parts(two different pdb structures previously solved contributing 55% and 15% of the target structure). I tried with molrep and phaser considering the first part(55%) as the search model but it turned out to no good solution which all clashes with symmetry related copies. If I assume the sp. gr. is not the case what else I can try. Any suggestion is well appreciated.
Thanks...
Raja

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Next message:
Subject: Question about the percentages of occupancy for dual conformations.
From: Jian Wu protease {- at -} GMAIL {- dot -} COM
Date: 2007-08-30