[ccp4bb] Expression of a Selenomethionine Variant in E. coli

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

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   - Peptide
   - Amino Acids

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: artificial tetramer
From: Fred ccp4bb {- dot -} list {- at -} GMAIL {- dot -} COM
Date: 2011-12-12

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Subject: Re: Expression of a Selenomethionine Variant in E. coli
From: Antony Oliver Antony {- dot -} Oliver {- at -} SUSSEX {- dot -} AC {- dot -} UK
Date: 2011-12-12

Subject: Expression of a Selenomethionine Variant in E. coli
From: Yibin Lin yybbll {- at -} GMAIL {- dot -} COM
Date: 2011-12-12

Hi List,

I want to preprare sel-met labeling protein. Could someone can tell me
if it is necessary to add DTT or BME to medium, which contains
sel-met, when growing cells?

Thanks in advance,

Yibin Lin

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: artificial tetramer
From: Fred ccp4bb {- dot -} list {- at -} GMAIL {- dot -} COM
Date: 2011-12-12

Next message:
Subject: Re: Expression of a Selenomethionine Variant in E. coli
From: Antony Oliver Antony {- dot -} Oliver {- at -} SUSSEX {- dot -} AC {- dot -} UK
Date: 2011-12-12