[ccp4bb] off-topic-Heat shock protein gets precipitated

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Date: 2007-08-30

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Date: 2007-08-30

Subject: off-topic-Heat shock protein gets precipitated
From: shivesh kumar 2shivesh {- at -} GMAIL {- dot -} COM
Date: 2007-08-30

Dear all
I am trying to purify HSP from C.trachomatis,a 57kDa protein for
crystallization.The yield is very good.The problem is autodegradation and
precipitation.I never reached to 1.5mg/ml concentration.There is a His-tag
at C-ter.I appreciate all the suggestins regarding get rid of at least
precipitation.Also appreciate worth suggestions from groups working with
chaperonins.Thanx in advance.
Shivesh kumar

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: MSE
From: Joe Krahn krahn {- at -} NIEHS {- dot -} NIH {- dot -} GOV
Date: 2007-08-30

Next message:
Subject: Re: MSE
From: Marko Hyvonen marko {- at -} CRYST {- dot -} BIOC {- dot -} CAM {- dot -} AC {- dot -} UK
Date: 2007-08-30