[ccp4bb] R/Rfree problem

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: His Purification
From: Mark J van Raaij mjvanraaij {- at -} CNB {- dot -} CSIC {- dot -} ES
Date: 2012-01-19

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Subject: Re: R/Rfree problem
From: Garib N Murshudov garib {- at -} MRC-LMB {- dot -} CAM {- dot -} AC {- dot -} UK
Date: 2012-01-19

Subject: R/Rfree problem
From: Á¶±âÁØ c-azna {- at -} KOREA {- dot -} AC {- dot -} KR
Date: 2012-01-19

Dear all,

I have a data, and it has R/Rfree problem.

The data could be processed as P212121 and P1.

The resolution was 2.9, and unit cell parameters were a=73.527, b=90.035,
c=237.980, ¥á=¥â=¥ì=90 and a=73.709, b=90.099, c=238.172, ¥á=89.939,
¥â=89.945, ¥ì=89.993 for P212121 and P1, respectively.

R/Rfree was 22.3/29.4 for P1 after several refinements.

However, I couldn¡¯t decrease R/Rfree below 30.0/37.6 for P212121.

There were almost no differences on monomer structure and crystal packing
between P212121 and P1 after MR, and the electron densities followed trace
of peptide chain well.

Can anybody suggest me about this problem?

Thank you.

Ki Joon Cho

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: His Purification
From: Mark J van Raaij mjvanraaij {- at -} CNB {- dot -} CSIC {- dot -} ES
Date: 2012-01-19

Next message:
Subject: Re: R/Rfree problem
From: Garib N Murshudov garib {- at -} MRC-LMB {- dot -} CAM {- dot -} AC {- dot -} UK
Date: 2012-01-19