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[ccp4bb] Rfree and unaccounted density

 

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CCP4bb <-- 2007 <-- September 2007 <-- 03 September 2007
Previous message:
Subject: Shelx install in ccp4iv6.0.2
From: Xue-Yuan Pei xyp20 {- at -} MOLE {- dot -} BIO {- dot -} CAM {- dot -} AC {- dot -} UK
Date: 2007-09-03
Next message:
Subject: chromatofocusing
From: James Fraser jamesfraser {- at -} BERKELEY {- dot -} EDU
Date: 2007-09-03


Subject: Rfree and unaccounted density
From: Vineet Gaur vineetgaur1982 {- at -} GMAIL {- dot -} COM
Date: 2007-09-03

Hi all

i m solving a structure at 2.2 A using MIR. rt now my Rfree is 30.55% and
Rcryst is 28.51%. i have already carried out B factor refinement n water has
also been picked up to 2.3 sigma cut off. now i m not able to refine the
structure any further.

The protein has been purified directly from the source. At this pt of
refinement i m able to see lot of unaccounted densities, far bigger to b
accounted by water or sugar, as we don't have any information abt the
possible molecules that can interact with this protein.
.
is this unaccounted density be the reason why i m not able to refine the
strucure?

thanx in advance

Vineet Gaur

CCP4bb navigation

CCP4bb <-- 2007 <-- September 2007 <-- 03 September 2007
Previous message:
Subject: Shelx install in ccp4iv6.0.2
From: Xue-Yuan Pei xyp20 {- at -} MOLE {- dot -} BIO {- dot -} CAM {- dot -} AC {- dot -} UK
Date: 2007-09-03
Next message:
Subject: chromatofocusing
From: James Fraser jamesfraser {- at -} BERKELEY {- dot -} EDU
Date: 2007-09-03



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