[ccp4bb] chromatofocusing

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Rfree and unaccounted density
From: Vineet Gaur vineetgaur1982 {- at -} GMAIL {- dot -} COM
Date: 2007-09-03

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Subject: Re: Shelx install in ccp4iv6.0.2
From: "P {- dot -} J {- dot -} Briggs" p {- dot -} j {- dot -} briggs {- at -} DL {- dot -} AC {- dot -} UK
Date: 2007-09-04

Subject: chromatofocusing
From: James Fraser jamesfraser {- at -} BERKELEY {- dot -} EDU
Date: 2007-09-03

I'm forwarding this to the listserve for someone in my lab who is
having trouble sending to the list. Replies to the list or directly
to Nat Echols (nat@lego.berkeley.edu) are greatly appreciated...

Not question about crystallography, but that's the ultimate goal:

Has anyone used a custom-made buffer for use with the PBE media sold by
GE? I found a bottle of Pharmacia PBE 94 and would like to use it, but
if I did I would use it frequently and on a relatively large scale, so
I'd rather not pay for the (expensive and proprietary) Polybuffer.

Alternately, has anyone done something similar with DEAE/CM media? I've
seen references to this (although I suspect the resolution won't be as
good), but not very good descriptions.

thanks,
Nat

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Rfree and unaccounted density
From: Vineet Gaur vineetgaur1982 {- at -} GMAIL {- dot -} COM
Date: 2007-09-03

Next message:
Subject: Re: Shelx install in ccp4iv6.0.2
From: "P {- dot -} J {- dot -} Briggs" p {- dot -} j {- dot -} briggs {- at -} DL {- dot -} AC {- dot -} UK
Date: 2007-09-04