Re: [ccp4bb] chromatofocusing

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: Shelx install in ccp4iv6.0.2
From: "P {- dot -} J {- dot -} Briggs" p {- dot -} j {- dot -} briggs {- at -} DL {- dot -} AC {- dot -} UK
Date: 2007-09-04

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Subject: Re: chromatofocusing
From: Cynthia Kinsland clk10 {- at -} CORNELL {- dot -} EDU
Date: 2007-09-04

Subject: Re: chromatofocusing
From: M T michelthe {- at -} GMAIL {- dot -} COM
Date: 2007-09-04

Any old references for "universal buffers"...

Johnson, and Lindsey, Analyst, 64 (1939).
Ellis, D. A., Nature, 191 (1961).
Prideaux, E. B. R. , and Ward, A. T. , J. Chem. Soc., 125, 426 (1924).
Britton, H. T. S. , and Robinson, R. A. , J. Chem. Soc., 1457 (1931).

But I never use these buffers myself and I don't know if these buffers
are suitable for your column, you have to check chemical
compatibility.
Most chromatofocusing common troubleshooting is protein precipitation
near isoelectric point, protein precipitation at unsupported pH and
CO2 degassing adsorbed by buffer at high pH and released at low pH.
Glycerol or betain can help you for protein precipitation and
degassing / N2 storing for CO2 releasing.
To optimize the resolution thin column and low flow rate are good choices.

Michel.

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Shelx install in ccp4iv6.0.2
From: "P {- dot -} J {- dot -} Briggs" p {- dot -} j {- dot -} briggs {- at -} DL {- dot -} AC {- dot -} UK
Date: 2007-09-04

Next message:
Subject: Re: chromatofocusing
From: Cynthia Kinsland clk10 {- at -} CORNELL {- dot -} EDU
Date: 2007-09-04