Re: [ccp4bb] chromatofocusing

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: chromatofocusing
From: M T michelthe {- at -} GMAIL {- dot -} COM
Date: 2007-09-04

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Subject: Why is RANGES restricted to <60 in TRUNCATE ?
From: "Juergen J {- dot -} Mueller" jjm {- at -} MDC-BERLIN {- dot -} DE
Date: 2007-09-04

Subject: Re: chromatofocusing
From: Cynthia Kinsland clk10 {- at -} CORNELL {- dot -} EDU
Date: 2007-09-04

You might look at this paper: http://www.ncbi.nlm.nih.gov/sites/
entrez?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12433100

They used DEAE and have given (in the supporting materials) a ton of
useful information.

Cynthia

On Sep 3, 2007, at 4:27 PM, James Fraser wrote:

> I'm forwarding this to the listserve for someone in my lab who is
> having trouble sending to the list. Replies to the list or directly
> to Nat Echols (nat@lego.berkeley.edu) are greatly appreciated...
>
> Not question about crystallography, but that's the ultimate goal:
>
> Has anyone used a custom-made buffer for use with the PBE media
> sold by
> GE? I found a bottle of Pharmacia PBE 94 and would like to use it,
> but
> if I did I would use it frequently and on a relatively large scale, so
> I'd rather not pay for the (expensive and proprietary) Polybuffer.
>
> Alternately, has anyone done something similar with DEAE/CM media?
> I've
> seen references to this (although I suspect the resolution won't be as
> good), but not very good descriptions.
>
> thanks,
> Nat

____________________
Cynthia Kinsland, Ph.D.
Cornell University
Protein Facility Director
607-255-8844

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: chromatofocusing
From: M T michelthe {- at -} GMAIL {- dot -} COM
Date: 2007-09-04

Next message:
Subject: Why is RANGES restricted to <60 in TRUNCATE ?
From: "Juergen J {- dot -} Mueller" jjm {- at -} MDC-BERLIN {- dot -} DE
Date: 2007-09-04