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[ccp4bb] MS for verification of protein constructs |
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CCP4bb navigationCCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999Subject: MS for verification of protein constructs From: Patrick Loll pat {- dot -} loll {- at -} DREXEL {- dot -} EDU Date: 2007-09-05 I wonder if anyone would care to share experiences/ideas/biases that relate to the use of mass spectrometry to verify the identity of protein constructs used for crystallization. Our experience with different MS facilities has been checquered. Specifically: What's the current thinking on the best approach to get masses for intact proteins of moderate size (say, 40 kD)? ESI-TOF? What kind of resolution should one hope to obtain in such cases (10E-04?) Any suggestions as to good facilities offering fee for service MS characterization are welcome (but should be shared off line, I think; continental US only). Thanks, Pat ------------------------------------------------------------------------ --------------- Patrick J. Loll, Ph. D. (215) 762-7706 Associate Professor FAX: (215) 762-4452 Department of Biochemistry & Molecular Biology Director, Biochemistry Graduate Program Drexel University College of Medicine Room 10-102 New College Building 245 N. 15th St., Mailstop 497 Philadelphia, PA 19102-1192 USA pat.loll@drexel.edu CCP4bb navigationCCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999 |
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