[ccp4bb] MS for verification of protein constructs

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: covalently bonded Ligand fit
From: "Debreczeni, Judit" Judit {- dot -} Debreczeni {- at -} ASTRAZENECA {- dot -} COM
Date: 2007-09-05

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Subject: Re: MS for verification of protein constructs
From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2007-09-05

Subject: MS for verification of protein constructs
From: Patrick Loll pat {- dot -} loll {- at -} DREXEL {- dot -} EDU
Date: 2007-09-05

I wonder if anyone would care to share experiences/ideas/biases that
relate to the use of mass spectrometry to verify the identity of
protein constructs used for crystallization. Our experience with
different MS facilities has been checquered.

Specifically:

What's the current thinking on the best approach to get masses for
intact proteins of moderate size (say, 40 kD)? ESI-TOF?
What kind of resolution should one hope to obtain in such cases
(10E-04?)

Any suggestions as to good facilities offering fee for service MS
characterization are welcome (but should be shared off line, I think;
continental US only).

Thanks,

Pat
------------------------------------------------------------------------
---------------
Patrick J. Loll, Ph. D. (215) 762-7706
Associate Professor FAX: (215) 762-4452
Department of Biochemistry & Molecular Biology
Director, Biochemistry Graduate Program
Drexel University College of Medicine
Room 10-102 New College Building
245 N. 15th St., Mailstop 497
Philadelphia, PA 19102-1192 USA

pat.loll@drexel.edu

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: covalently bonded Ligand fit
From: "Debreczeni, Judit" Judit {- dot -} Debreczeni {- at -} ASTRAZENECA {- dot -} COM
Date: 2007-09-05

Next message:
Subject: Re: MS for verification of protein constructs
From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2007-09-05