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Re: [ccp4bb] Freezing crystal |
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CCP4bb navigationCCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999Subject: Re: Freezing crystal From: "Edward A {- dot -} Berry" BerryE {- at -} UPSTATE {- dot -} EDU Date: 2012-02-08 Bosch, Juergen wrote: > Hi Dirk, > > I remember a neat paper don't recall who wrote it. I think it was in Acta D where the > authors made a tiny probe the size of an elongated crystal glued to a [/Advertisement on] > Hampton loop [/Advertisement off]. The probe was a temperature sensor and they recorded > the cooling rate under different methods. The winner as far as I recall was freezing in > liquid propane for the lack of the missing gas layer, but the second best method was LN2. > Propane for whatever reason has gone extinct in certain areas of the world :-) . I'll try > to find that reference but perhaps somebody else on this highly educated board knows which > paper I'm referring to. I want to say it was published around 2004-2006. > Not highly educated, but I remember hearing Haken Hope talk about this experiment at a workshop at SSRL- cooling a thermocouple or thermister in the cold stream vs in LN2. Maybe described here: Cryocrystallography of biological macromolecules: a generally applicable method. Hope H. Acta Crystallogr B. 1988 Feb 1;44 ( Pt 1):22-6. BTW I don't thing the greater cooling rate with ethane/propane is due to greater heat capacity so much as the fact that LN2 is used at it's boiling point: heat capacity is irrelevant since it can't absorb any more heat as a liquid, latent heat of vaporization is the reelevant parameter, but once it is vaporized the gas has low heat capacity and thermal conductivity. The liquid hydrocarbons are prepared by chilling to around their freezing point (hydrocarbon slush) so can absorb a lot of heat before any gas forms. > Jürgen > > On Feb 7, 2012, at 11:12 AM, Dirk Kostrewa wrote: > >> Dear Jürgen, >> >> Am 07.02.12 16:58, schrieb Bosch, Juergen: >> >>> Then one last remark, LN2 versus cryo-stream freeze. Dipping in LN2 >>> leads to a quicker freeze of your material. >> >> >> Are you sure? There was a publication by Warkentin et al. [1] about a >> cold gas layer above liquid nitrogen that reduces the expected cooling >> rate a lot! >> My very personal experience is, that cryo-cooling in the N2-stream >> worked better for me than in LN2 in a variety of projects - but the >> reason could just be me ;-) >> >> Best regards, >> >> Dirk. >> >> [1] Matthew Warkentin, Viatcheslav Berejnov, Naji S Husseini, and Robert >> E Thorne: "Hyperquenching for protein cryocrystallography", J. Appl. >> Crystallogr., 39, 805-811 (2006) >> >> -- >> >> ******************************************************* >> Dirk Kostrewa >> Gene Center Munich >> Department of Biochemistry >> Ludwig-Maximilians-Universität München >> Feodor-Lynen-Str. 25 >> D-81377 Munich >> Germany >> Phone: +49-89-2180-76845 >> Fax: +49-89-2180-76999 >> E-mail:kostrewa@genzentrum.lmu.de >> WWW:www.genzentrum.lmu.de >> ******************************************************* > > ...................... > Jürgen Bosch > Johns Hopkins University > Bloomberg School of Public Health > Department of Biochemistry & Molecular Biology > Johns Hopkins Malaria Research Institute > 615 North Wolfe Street, W8708 > Baltimore, MD 21205 > Office: +1-410-614-4742 > Lab: +1-410-614-4894 > Fax: +1-410-955-2926 > http://web.mac.com/bosch_lab/ > > > > CCP4bb navigationCCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999 |
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