Re: [ccp4bb] surface residue mutation

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: DNA in coot
From: "William G {- dot -} Scott" wgscott {- at -} UCSC {- dot -} EDU
Date: 2012-02-15

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Subject: Re: DNA in coot
From: Tim Gruene tg {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2012-02-15

Subject: Re: surface residue mutation
From: Enrico Stura estura {- at -} CEA {- dot -} FR
Date: 2012-02-15

Dear All,

One of the most efficient methods to change space group and packing
without having to change
the sequence is to change the length of N and/or C terminal tags.

An example that I am familiar with is given by the following PDB codes.

1JIZ, 1RMZ, 1JK3, 1UTT, 1UTZ, 2WOA, 2W0D, 1ROS, 1OS9, 3BA0

It includes 1 surface residue mutation, but the rest are small variations
in length.

Complexation with any ligand that may protrude is also likely to work.

Enrico.

On Wed, 15 Feb 2012 01:35:36 +0100, Bernhard Rupp (Hofkristallrat a.D.)
wrote:

>
> http://services.mbi.ucla.edu/SER/
>
>
> but no space group predictions are possible. BR
>
>
> From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
> Prem
> Kaushal
> Sent: Tuesday, February 14, 2012 3:36 PM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: [ccp4bb] surface residue mutation
>
>
>
>
>
> Hi
>
> We have a protein that crystallized in P21212 space group. We are looking
> for some different crystal forms. We tried few things did not work. Now
> we
> are thinking to mutate surface residues. Anybody aware of any software
> which
> can predict the mutations that might help in crystallizing protein in
> different space group, please inform me.
>
> Thanks in advance
>
> Prem
>
>

--
Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office
Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab
LTMB, SIMOPRO, IBiTec-S, CE Saclay, 91191 Gif-sur-Yvette, FRANCE
http://www-dsv.cea.fr/en/institutes/institute-of-biology-and-technology-saclay-ibitec-s/unites-de-recherche/department-of-molecular-engineering-of-proteins-simopro/molecular-toxinology-and-biotechnology-laboratory-ltmb/crystallogenesis-e.-stura
http://www.chem.gla.ac.uk/protein/mirror/stura/index2.html
e-mail: estura@cea.fr Fax: 33 (0)1 69 08 90 71

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: DNA in coot
From: "William G {- dot -} Scott" wgscott {- at -} UCSC {- dot -} EDU
Date: 2012-02-15

Next message:
Subject: Re: DNA in coot
From: Tim Gruene tg {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2012-02-15